Isolation and characterization of a novel fucoganglioside of human erythrocyte membranes.
作者： M Powell , S Hakomori , K Watanabe
DOI: 10.1016/S0021-9258(17)34271-0
关键词:
摘要: A slow migrating monosialosyl ganglioside with blood group H activity was isolated from human erythrocyte membranes and identified as monosialosylceramide decasaccharide. Its structure determined by degradation exo- endoglycosidases, mass spectrometric characterization of a nonasaccharide liberated endo-beta-galactosidase, methylation analysis spectrometry permethylated glycolipids before after enzymatic degradation. The contains two oligosaccharide chains branched through GlcNAcbeta1 leads to 6(GlcNAcbeta1 3) Gal structure, the terminal 1 6-linked fucosylated, whereas that 3-linked sialosylated seen below.
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