Laser capture microdissection and real-time reverse transcriptase/ polymerase chain reaction of bronchiolar epithelium after bleomycin.

摘要: Terminal airways are affected in many lung diseases and toxic inhalations. To elucidate the changes terminal these diverse situations it will be helpful to profile quantify gene expression bronchiolar epithelium. We used laser capture microdissection (LCM) collect epithelial cells from frozen sections of lungs mice subjected intratracheal bleomycin. The RNA was for analysis select messenger RNAs (mRNAs) by quantitative real-time polymerase chain reaction (PCR). In parallel, we PCR analyze mRNAs whole-lung homogenates prepared other given found reductions Clara cell–specific protein keratinocyte growth factor receptor both epithelium 7 d after contrast, transforming (TGF)- α mRNA reduced but TGF- not changed, whereas ...