Detection of HIV-1 DNA in needle/syringes, paraphernalia, and washes from shooting galleries in Miami: a preliminary laboratory report.

摘要: Shared use of injection equipment (needle/syringes), registering, booting, and backloading are practices among drug users (IDUs) that increase the risk for transmission human immunodeficiency virus type 1 (HIV-1). The sharing paraphernalia (including cookers cottons) washwater rinsing used needle/syringes dissolving drugs could be potential sources secondary HIV-1. Laboratory rinses were made from needle/syringes, cottons, obtained shooting galleries, washwaters galleries in Miami. Three analyzed antibodies to HIV-1 proteins detected by using Western blot DNA was nested polymerase chain reaction (PCR) specific gag envelope genes Antibodies 12 (52%) 23 visibly contaminated three (18%) 17 (14%) 21 cookers, one (6%) washwaters. No laboratory clean needles. Using PCR followed Southern confirmation amplified targets, gene 16 (84%) 19 (85%) 20 needle/syringes. We DNA, respectively, (27%) four (36%) 11 six (46%) seven (54%) 13 five (38%) 10 (67%) 15 galleries. These results indicate might present as well at Reduction risks exposure IDUs may require modification behaviors ancillary act injection, such common washwater.