Aromatic hydroxylation of phenylalanine as an assay for hydroxyl radicals: application to activated human neutrophils and to the heme protein leghemoglobin.
作者： Harparkash Kaur , Ingrid Fagerheim , Martin Grootveld , Alain Puppo , Barry Halliwell
DOI: 10.1016/0003-2697(88)90456-3
关键词:
摘要: Attack of hydroxyl radical (.OH), generated by a Fenton system at physiological pH, upon L-phenylalanine produces three isomeric tyrosines, o-tyrosine (2-hydroxyphenylalanine), m-tyrosine (3-hydroxyphenylalanine), and p-tyrosine (4-hydroxyphenylalanine). These may be separated high-performance liquid chromatography measured using an electrochemical detector. Since is relatively nontoxic, it proposed that generation these tyrosines from phenylalanine can used as assay for .OH in biological systems. The use the to measure production leghemoglobin (plus H2O2) activated human neutrophils described. No was observed unless source iron ions added reaction mixture, which suggests cells do not release "promoter" superoxide hydrogen peroxide.
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