Studies on the utilisation of carbohydrates and krebs cycle intermediates by Rhizobia, using an agar plate method

摘要: An agar plate method for the testing of substrate utilisation is described, and applied to 108 strains bacteria belonging generaRhizobium andAgrobacterium. With this method, can be observed without misleading results due amino acids other substances present in medium. Major differences carbohydrates by tested species are apparent. In general, fast-growing root-nodule agrobacteria were similar reaction, giving vigorous growth with most carbon sources tested. Slow-growing more specific their requirements, utilising only glucose, sodium citrate, xylose, mannitol, arabinose, galactose fructose. Only six utilised dextrin. The related previous studies on rhizobial taxonomy, value carbohydrate as a diagnostic feature discussed.