Molecular cloning of the MCP-3 chemokine gene and regulation of its expression.

摘要: We have isolated a cDNA (NC28) transcribed from mRNA which is transiently induced in U937 promonocytic cells by PMA and super-induced cycloheximide. NC28 encodes new member of the chemokine family, MCP-3, recently purified MG-63 osteosarcoma Van Damme et al. [1]. The MCP-3 protein sequence shows 74% identity with human monocyte chemoattractant 1 (MCP-1) and, like MCP-1, recombinant chemotactic activity for monocytes but not neutrophils. However secreted differs MCP-1 being N-glycosylated. 3' noncoding regions mRNAs are more diverged (44%), allowing specific probes to be made, indicating that two genes evolutionarily distant. Sequence comparisons suggest may homologue mouse MARC gene [2], arose duplication event before mammalian radiation. Both expressed PBMC, principally monocytes, at levels 2-4 times mRNA. However, while also high fibroblast or astrocytoma cell lines after IL-1 TNF stimulation, only very low these cells. cellular origin thus restricted than MCP-1. In our experiments on LPS consistent inducer mRNAs. some experiments, it actually decreases mRNAs, concomitantly increasing IL-6 TNF-alpha levels. Levels PBMC both increased IFN-gamma, although induced. They PHA-P decreased, most cases, IL-13 [3]. co-ordinately regulated response number inducing inhibitory agents, manner differing several respects other monokines such as IL-6.