Qualitative and Quantitative Immunoenzymatic Techniques

作者: Stratis Avrameas

DOI: 10.1007/978-1-4684-4046-1_4

关键词:

摘要: Because an enzyme can be revealed and also accurately determined by appropriate procedures, enzyme-labelled antigens antibodies used to localize, detect or measure constituents of biological interest (1,2). Such immunoenzyme techniques applied in several ways both basic research biomedical diagnosis. 1) Localization cellular constituents; 2) quantitation present fluids cells; 3) detection small amounts antigen-antibody immune precipitates.

参考文章(23)
J.G. Streefkerk, A.M. Deelder, Serodiagnostic application of immunohistoperoxidase reactions on antigen-coupled agarose beads Journal of Immunological Methods. ,vol. 7, pp. 225- 235 ,(1975) , 10.1016/0022-1759(75)90020-4
S. Avrameas, Brigitte Guilbert, A method for quantitative determination of cellular immunoglobulins by enzyme‐labeled antibodies European Journal of Immunology. ,vol. 1, pp. 394- 396 ,(1971) , 10.1002/EJI.1830010518
Eva Engvall, Peter Perlmann, Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G Immunochemistry. ,vol. 8, pp. 871- 874 ,(1971) , 10.1016/0019-2791(71)90454-X
T. E. MASON, R. F. PHIFER, S. S. SPICER, R. A. SWALLOW, R. B. DRESKIN, An immunoglobulin-enzyme bridge method for localizing tissue antigens. Journal of Histochemistry and Cytochemistry. ,vol. 17, pp. 563- 569 ,(1969) , 10.1177/17.9.563
Jean-Luc Guesdon, Stratis Avrameas, An immunoenzymatic method for measuring low concentrations of antigens by single radial diffusion Immunochemistry. ,vol. 11, pp. 595- 598 ,(1974) , 10.1016/0019-2791(74)90251-1
S. AVRAMEAS, T. TERNYNCK, J.-L. GUESDON, Coupling of Enzymes to Antibodies and Antigens Scandinavian Journal of Immunology. ,vol. 8, pp. 7- 23 ,(1978) , 10.1111/J.1365-3083.1978.TB03880.X
PAUL K. NAKANE, AKIRA KAWAOI, Peroxidase-labeled antibody. A new method of conjugation Journal of Histochemistry and Cytochemistry. ,vol. 22, pp. 1084- 1091 ,(1974) , 10.1177/22.12.1084
J L Guesdon, T Ternynck, S Avrameas, The Use of Avidin-Biotin Interaction in Immunoenzymatic Techniques Journal of Histochemistry and Cytochemistry. ,vol. 27, pp. 1131- 1139 ,(1979) , 10.1177/27.8.90074