A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance.

摘要: The tetracycline resistance regulatory gene (tetR) of transposon Tn10 was analyzed by a combination methods involving fusion and cloning. This is located on 695-base pair HincII DNA fragment near the center Tn10. direction transcription opposite to that neighboring tetA, which encodes TetA protein. product tetR (the TetR protein) has molecular weight 23,000. tet-R-lacZ fusions encode beta-galactosidases are membrane bound, indicating protein itself associated. Mutants defective in result constitutive resistance, but level reduced. Expression induced tetracycline; absence tetracycline, turns off its own synthesis.