Transcriptomic identification of adh1b as a novel candidate gene for obesity and insulin resistance in human adipose tissue in mexican americans from the veterans administration genetic epidemiology study (vages)

摘要: Type 2 diabetes (T2D) is a complex metabolic disease that more prevalent in ethnic groups such as Mexican Americans, and strongly associated with the risk factors obesity insulin resistance. The goal of this study was to perform whole genome gene expression profiling adipose tissue detect common patterns regulation We used phenotypic genotypic data from 308 American participants Veterans Administration Genetic Epidemiology Study (VAGES). Basal fasting RNA extracted biopsies subset 75 unrelated individuals, generated on Illumina BeadArray platform. number probes significant above baseline approximately 31,000. performed multiple regression analysis all 15 traits. Adipose had 3,012 genes significantly traits interest (false discovery rate, FDR ≤ 0.05). significance changes select 52 (FDR 10-4) across Gene sets/Pathways identified one gene, alcohol dehydrogenase 1B (ADH1B) enriched (P < 10-60) prime candidate for involvement relevant pathways. BeadChip derived ADH1B consistent quantitative real time PCR data. observed inverse correlations waist circumference (2.8 x 10-9), BMI (5.4 10-6), plasma 0.001). These findings are central role resistance provide evidence novel genetic regulatory mechanism human diseases related these