Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS

摘要: Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further fed-batch fermentation. The purified 62 kDa monomeric optimally active at pH 2.5 and 60 °C retained activity over wide range temperature (40–80 °C) (2.0–6.0) with half-life 11.3 min 80 °C. kinetic K m, V max, cat, cat/K m the pure were 0.21 mM, 131.58 nmol mg−1 s−1, 1.64 × 103 7.81 106 M−1 respectively. enzyme fairly stable presence pepsin under physiological conditions. It stimulated Ca+2, Mg+2 Mn+2, but inhibited Zn+2, Cu+2, Fe+2, Pb+2, Ba+2 surfactants. can be applied dephytinizing animal feeds, baking industry.