Involvement of methylation-associated silencing of formin 2 in colorectal carcinogenesis.

摘要: AIM To investigate whether promoter methylation is responsible for the silencing of formin 2 (FMN2) in colorectal cancer (CRC) and to analyze association between FMN2 CRC. METHODS We first identified expression levels large-scale human CRC datasets, including GEO TCGA, analyzed relationship levels. Then, four CpG regions adjacent were assessed by MethylTarget™ assays cells paired tumor samples nontumor tissue samples. Furthermore, we inhibited DNA with 5-Aza-2'-deoxycytidine qRT-PCR. Last, patterns clinical indicators was analyzed. RESULTS A statistically significant downregulation datasets found. Subsequent analysis showed that a high frequency hypermethylation occurred gene tissues; operating characteristic curve revealed had good capability discriminating (AUC = 0.8432, P 60 years old colon tissue. CONCLUSION may be an important early event CRC, most likely playing critical role initiation, can serve as ideal diagnostic biomarker elderly patients early-stage cancer.