摘要: The voltage dependence of K+, Na+, and Ca2+ channels is brought about by a sensor that moves ∼12–13 e0 across the entire electric field (Schoppa et al., 1992; Hirschberg 1996; Noceti 1996). In case Shaker K+ channel it known which residues are responsible for this large amount gating charge. This was found measuring total charge movement per after each putative charged (basic or acidic) were neutralized one (Aggarwal MacKinnon, Seoh These studies revealed four most extracellularly located basic S4 segment (R362, R365, R368, R371) intracellular acidic residue in S2 (E293) major contributors to movement. simple assumption all these move accounts more than measured means at least some only partial within field.
rupress.org
jgp.org参考文章(36)
Paul R. Schimmel, Charles R. Cantor, Techniques for the study of biological structure and function W. H. Freeman. ,(1980)
Benoit Roux, What can be deduced about the structure of Shaker from available data Novartis Foundation symposium. ,vol. 245, pp. 84- 108 ,(2002) , 10.1002/0470868759.CH7
Albert Cha, Gregory E. Snyder, Paul R. Selvin, Francisco Bezanilla, Atomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy Nature. ,vol. 402, pp. 809- 813 ,(1999) , 10.1038/45552
Christopher Miller, Robert O. Blaustein, Philip A. Cole, Carole Williams, Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel. Nature Structural & Molecular Biology. ,vol. 7, pp. 309- 311 ,(2000) , 10.1038/74076
K. S. Glauner, L. M. Mannuzzu, C. S. Gandhi, E. Y. Isacoff, Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel Nature. ,vol. 402, pp. 813- 817 ,(1999) , 10.1038/45561
O.S Baker, H.P Larsson, L.M Mannuzzu, E.Y Isacoff, Three Transmembrane Conformations and Sequence-Dependent Displacement of the S4 Domain in Shaker K+ Channel Gating Neuron. ,vol. 20, pp. 1283- 1294 ,(1998) , 10.1016/S0896-6273(00)80507-3
María del Mar Martínez-Senac, Senena Corbalán-García, Juan C Gómez-Fernández, None, The Structure of the C-Terminal Domain of the Pro-Apoptotic Protein Bak and Its Interaction with Model Membranes Biophysical Journal. ,vol. 82, pp. 233- 243 ,(2002) , 10.1016/S0006-3495(02)75390-3
Dejian Ren, Betsy Navarro, Haoxing Xu, Lixia Yue, Qing Shi, David E Clapham, None, A Prokaryotic Voltage-Gated Sodium Channel Science. ,vol. 294, pp. 2372- 2375 ,(2001) , 10.1126/SCIENCE.1065635
Seema K. Tiwari-Woodruff, Meng-chin A. Lin, Christine T. Schulteis, Diane M. Papazian, Voltage-dependent structural interactions in the Shaker K(+) channel. The Journal of General Physiology. ,vol. 115, pp. 123- 138 ,(2000) , 10.1085/JGP.115.2.123
Sang-Ah Seoh, Daniel Sigg, Diane M Papazian, Francisco Bezanilla, Voltage-Sensing Residues in the S2 and S4 Segments of the Shaker K+ Channel Neuron. ,vol. 16, pp. 1159- 1167 ,(1996) , 10.1016/S0896-6273(00)80142-7