Practical application of transcriptional sequencing for GC-rich templates.

摘要: Transcriptional sequencing (TS) is a method that differs considerably from conventional methods. These differences include the use RNA polymerases with rNTPs and 3′-dNTPs as substrates terminators respectively, initiation double stranded promoters on templates of ds-DNA. We used TS in an attempt to sequence 33 clones whose electropherogram peaks suddenly became absent or weak All reactions overcame difficulty problematic target regions clones. Therefore, can be applied not only GC-rich regions, but also whole genome sequences high GC content.