Isolation and characterization of the products of anoxic irradiation of d(CpGpTpA)

摘要: An improved method for separating the products of DNA oligomers irradiated in aqueous solution has been devised. Altogether 39 were isolated from tetramer d(CpGpTpA) X-irradiated dilute anoxic solution. Of these, 16, including most major products, identified through use proton nmr spectroscopy. The fall into four categories: (1) base (2) strand scission (3) modifications and (4) tandem lesion. A lesion which methyl carbon atom thymine is covalently linked to guanine C8 was produced larger yield than any simple modifications.