Methylation status of the APC and RASSF1A promoter in cell-free circulating DNA and its prognostic role in patients with colorectal cancer.

摘要: DNA methylation is the most frequent epigenetic alteration. Using methylation-specific polymerase chain reaction (MSP), status of adenomatous polyposis coli (APC) and Ras association domain family 1 isoform A (RASSF1A) genes was examined in cell-free circulating from 155 plasma samples obtained patients with early advanced colorectal cancer (CRC). APC RASSF1A hypermethylation frequently observed both disease, significantly associated a poorer disease outcome. The promoters investigated CRC. MSP, promoter blood CRC, 88 whom had operable CRC (oCRC) 67 metastatic (mCRC). frequency oCRC 33%. Methylated older age (P=0.012), higher stage (P=0.014) methylated (P=0.050). mCRC 53.7%. In these patients, (P=0.016). 25%. (P=0.021). 44.8%. moderate differentiation high levels carcinoembryonic antigen (P=0.023) Patients an unmethylated gene better survival (81±5 vs. 27±4 months, P<0.001) (37±7 15±3 P<0.001), compared APC. (71±6 46±8 (28±4 16±3 than RASSF1A. significant correlations between may be indicative prognostic role for which requires additional testing larger studies.