Increased DNA vaccine delivery and immunogenicity by electroporation in vivo.

摘要: DNA vaccines have been demonstrated to be potent in small animals but are less effective primates. One limiting factor may inefficient uptake of by cells situ. In this study, we evaluated whether cellular was a significant barrier efficient transfection vivo and subsequent induction immune responses. For purpose, used the technique electroporation facilitate delivery vivo. This technology shown substantially increase cells, resulting increased expression elevated The potency weakly immunogenic hepatitis B surface Ag vaccine mice, as seen more rapid onset higher magnitude anti-hepatitis Abs. addition, immunogenicity HIV gag Ab titers, substantial reduction dose required induce an response, CD8+ T cell Finally, responses were enhanced against both components combination env guinea pigs rabbits. Therefore, is vivo, appears able overcome barrier.