Simple and Rapid Method for Measuring Turbidity in Gels and Sols from Milk Whey Protein

作者: NAOFUMI KITABATAKE , ETSUSHIRO DOI , YOH-ICHI KINEKAWA

DOI: 10.1111/J.1365-2621.1994.TB08124.X

关键词:

摘要: Protein isolates prepared from acid whey concentrate and cheese concentrate, β-lactoglobulin were studied. Turbidity of samples was measured using a 96-well microplate microreader. This method allowed many (>100) small (< 250 μL) to be treated at the same time. At low ionic strength neutral alkaline pH, transparent after heating. Transparent gels could also in this region with amount NaCl.

参考文章(16)
THE HYDROGEN ION DISSOCIATION CURVE OF β-LACTOGLOBULIN Journal of Biological Chemistry. ,vol. 142, pp. 803- 822 ,(1942) , 10.1016/S0021-9258(18)45079-X
R. R. Zall, Sources and Composition of Whey and Permeate Whey and lactose processing.. pp. 1- 72 ,(1992) , 10.1007/978-94-011-2894-0_1
William J. Leonard, Kamala Kant Vijai, Joseph F. Foster, A Structural Transformation in Bovine and Human Plasma Albumins in Alkaline Solution as Revealed by Rotatory Dispersion Studies Journal of Biological Chemistry. ,vol. 238, pp. 1984- 1988 ,(1963) , 10.1016/S0021-9258(18)67930-X
NAOFUMI KITABATAKE, YOSHINORI TANI, ETSUSHIRO DOI, Rheological Properties of Heat-Induced Ovalbumin Gels Prepared by Two-step and One-step Heating Methods Journal of Food Science. ,vol. 54, pp. 1632- 1638 ,(1989) , 10.1111/J.1365-2621.1989.TB05176.X
A.H. CLARK, F.J. JUDGE, J.B. RICHARDS, J.M. STUBBS, A. SUGGETT, Electron microscopy of network structures in thermally-induced globular protein gels. International Journal of Peptide and Protein Research. ,vol. 17, pp. 380- 392 ,(2009) , 10.1111/J.1399-3011.1981.TB02005.X
Naofumi KITABATAKE, Hajime HATTA, Etsushiro DOI, Heat-induced and Transparent Gel Prepared from Hen Egg Ovalbumin in the Presence of Salt by a Two-step Heating Method Agricultural and biological chemistry. ,vol. 51, pp. 771- 778 ,(1987) , 10.1271/BBB1961.51.771
Taihei Koseki, Naofumi Kitabatake, Etsushiro Doi, Irreversible thermal denaturation and formation of linear aggregates of ovalbumin Food Hydrocolloids. ,vol. 3, pp. 123- 134 ,(1989) , 10.1016/S0268-005X(89)80022-0
John E. Kinsella, Charles V. Morr, Milk proteins: physicochemical and functional properties Critical Reviews in Food Science and Nutrition. ,vol. 21, pp. 197- 262 ,(1984) , 10.1080/10408398409527401
Robert Townend, R. J. Winterbottom, Serge N. Timasheff, Molecular Interactions in β-Lactoglobulin. II. Ultracentrifugal and Electrophoretic Studies of the Association of β-Lactoglobulin below its Isoelectric Point2 Journal of the American Chemical Society. ,vol. 82, pp. 3161- 3168 ,(1960) , 10.1021/JA01497A045
M.E. Mangino, J.H. Kim, J.A. Dunkerley, J.G. Zadow, Factors important to the gelation of whey protein concentrates Food Hydrocolloids. ,vol. 1, pp. 277- 282 ,(1987) , 10.1016/S0268-005X(87)80015-2