A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

摘要: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase that form base pairs during DNA polymerase- mediated replication or RNA polymerase-mediated transcription RNA. In this manner, the nucleobases can be introduced in a site-specific way into oligonucleotides (single double stranded RNA), where they provide for cleavage, reactive linker than undergo functionalization with cargo -bearing reagent by means reaction primary amino group click chemistry an alkyne linker.