Genomic and proteomic analysis of chemoresistance in breast cancer cell lines: MCF-7 and MDA-MB-231

摘要: In this study, cisplatin-resistant adenocarcinoma breast cancer cell lines, labeled MCF-7/R6 and MDA-MB-231/R2 were established by 6 replicative sequential treatment of low doses (1 micromolar) over high (30 cisplatin chemoresistance was evaluated as apoptotic response to 30 micromolar for 48 hours. Throughout the study parental cells used control. RNA isolated conduct microarray analyze 48000 gene probes (Illumina Human WG-6 BeadChip). Gene expression data analyzed MATLAB 2009 identification differentially expressed genes further studied proteomic analysis understand drug resistance mechanisms. Our indicated that transcription resistant marker genes, such GSTP1 ABCB6 upregulated in line. Both intrinsically line: MDA-MB-231 acquired similar activating NF- kappa-B pathway but not MAP kinase pathway. line NF-kappa-B transactivated through p50 subunit, well translocation Foxo3a factor into nucleus. Genes responsible death, Foxo family MAPK levels found change significantly at protein genetic level cells. Phosphorylation Foxo1 cells, indicating apoptosis. Moreover, cycle, tumor suppressor estrogen receptor be altered clearly identify several proteins pathways which have distinct roles mechanism. This knowledge can validated a clinical setting.