Bacterial replicases and related polymerases.

摘要: Bacterial replicases are complex, tripartite replicative machines. They contain a polymerase, Pol III, β(2) processivity factor and DnaX complex ATPase that loads onto DNA chaperones III the newly loaded β(2). Many bacteria encode both full length τ shorter γ form of by variety mechanisms. The polymerase catalytic subunit α, contains PHP domain not only binds to prototypical ɛ Mg(2+)-dependent exonuclease, but also second Zn(2+)-dependent proofreading at least in some bacteria. Replication chromosomes low GC Gram-positive require two IIIs, one which, DnaE, appears extend RNA primers short distance before handing product off major replicase, PolC. Other (ImuC) apparently replaces V, required for induced mutagenesis E. coli. Approaches permit simultaneous biochemical screening all components bacterial promise inhibitors specific protein targets reaction stages.