Glomerular basement membrane discontinuities. Scanning electron microscopic study of acellular glomeruli.

摘要: Glomerulonephritides which develop necrotizing and crescentic lesions usually have glomerular basement membrane (GBM) disruptions when carefully examined by light microscopy or transmission electron microscopy. Despite numerous excellent detailed ultrastructural investigations of GBM discontinuities, a complete appreciation their actual number, appearance, distribution within glomerulus has been difficult to achieve reconstruction two-dimensional microscopic images. Selective removal podocytes sequence lytic solubilization procedures developed exposes any structural alteration the direct examination scanning A case idiopathic, immune-complex-negative, focal-segmental glomerulonephritis studied this technique, permitting three-dimensional visualization defects result in free communication between vascular urinary spaces. These were distinctive frequency an affected lobule, variable size, sharply demarcated edges. Application technique human renal biopsies is capable enhancing our understanding morphologic alterations occurring glomerulonephritis.