Modulation of gap junctions in senescent endothelial cells

摘要: Abstract Gap junction-mediated intercellular communication (GJIC) was decreased in senescent human umbilical vein endothelial cells (HUVEC), as detected by Gap-Frap studies. The molecular basis of this reduction and the effects calcium ionophore A23187 epidermal growth factor (EGF) on young old HUVEC have been investigated. Northern Western analyses reveal that levees both cx43 (connexin 43) messenger RNA protein decline age vitro . While responded immediately to increases intracellular concentrations, only produced a dose-dependent decrease cell coupling response addition exogenous EGF. down-regulation mRNA levels suggests GJIC might play role aging process. inability down-regulate gap junctions EGF reflects defect regulatory mechanism junction activity cells.