The role of annexin 2 in osteoblastic mineralization.

摘要: While the basic cellular contributions to bone differentiation and mineralization are widely accepted, regulation of these processes at intracellular level remains inadequately understood. Our laboratory recently identified annexin 2 as a protein involved in osteoblastic mineralization. Annexin was overexpressed twofold SaOSLM2 cells fusion with green fluorescent protein. The overexpression led an increase alkaline phosphatase activity well data suggest that does not result from increased transcript or levels; therefore we evaluated mechanism action. We determined both were localized membrane microdomains called lipid rafts cells. resulted associated cholesterol-dependent manner. Furthermore, disruption cholesterol sequestering agent reduction expression by specific antisense oligonucleotides each diminished Therefore, intact containing appear be important for may facilitate process.