Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation-Robotic Liquid Micro-Extraction Surface Analysis-Liquid Chromatography Mass Spectrometry
作者： William Griffiths
DOI: 10.1101/450973
关键词:
摘要: In the current study we describe how have exploited on-tissue enzyme assisted derivatisation with micro-Liquid-Extraction-for-Surface-Analysis and nano-liquid chromatography-mass spectrometry for imaging of oxysterols in rodent brain. We find 24S-hydroxycholesterol (24S-HC) to be most abundant oxysterol all brain regions being at highest concentration striatum thalamus lowest levels grey matter cerebellum. are also able, first time, definitively identify 3β,7α-dihydroxycholest-5-en-(25R)26-oic acid brain, where it is confirm that 24S-HC essentially absent from CYP46A1 knockout mouse replaced by lower 25-hydroxycholesterol.
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