Azole substituted oligonucleotides promote antiparallel triplex formation at non-homopurine duplex targets

摘要: The ability of certain azole substituted oligodeoxy-ribonucleotides to promote antiparallel triple helix formation with duplex targets having CG or TA interruptions in the otherwise homopurine sequence was examined. 2'-Deoxyribonucleosides azoles, which include pyrazole, imidazole, 1,2,4-triazole and 1,2,3,4-tetrazole were synthesized using stereo-specific sodium salt glycosylation procedure. These nucleosides successfully incorporated solid-support, phosphoramidite chemistry, into oligonucleotides designed interact non-homopurine targets. interaction these modified all four possible base pairs evaluated compared similar data for a series natural oligonucleotides. containing simple azoles enhanced triplex forming considerably at Binding inversion sites particularly noteworthy, compare favorably unmodified binding as well pairs. selectivity exhibited by is suggestive pair specific interactions. Thus, during this study show considerable promise efforts develop generalized sequences.