Cytokine-induced gene expression of a neutrophil chemotactic factor/IL-8 in human hepatocytes.

摘要: The liver participates in inflammation via the elaboration of acute phase proteins from hepatocytes response to IL-1, TNF-alpha, and IL-6/INF-beta 2/hepatocyte-stimulating factor. In addition, some inflammatory states are characterized by leukocyte infiltrates. Here we demonstrate that human hepatocyte lines capable expressing mRNA biologic activity for a neutrophil chemotactic factor (NCF)/IL-8 mediators IL-1 alpha, beta, TNF. Two hepatoma cell (SK-Hep Hep-G2) displayed time- dose-dependent increase steady state levels NCF/IL-8 secretion TNF IL-1. Neutralizing antibody inhibited hepatocyte-derived 88%. contrast TNF, did not respond LPS or IL-6 within time dose parameters used above. Although expression (1.8 kb) was first detectable between 1 2 h poststimulation, significant bioactivity observed until about 4 h. Heat-inactivated (100 degrees C, 30 min) cytokine failed induced synthesis, cotreatment cells with cycloheximide super-induced while inhibiting production bioactivity. Thus, is primary induction phenomenon. Our data stimulus specific suggest cell-to-cell communication circuits may be important neutrophil-mediated processes liver.