Role of NFKB2 on the early myeloid differentiation of CD34+ hematopoietic stem/progenitor cells
作者： Greice Andreotti De Molfetta , Dalila Lucíola Zanette , Rodrigo Alexandre Panepucci , Anemarie Ramos Dinarte dos Santos , Wilson Araújo da Silva
DOI: 10.1016/J.DIFF.2010.07.004
关键词:
摘要: To better understand the early events regulating lineage-specific hematopoietic differentiation, we analyzed transcriptional profiles of CD34+ human stem and progenitor cells (HSPCs) subjected to differentiation stimulus. were cultured for 12 40h in liquid cultures with supplemented media favoring myeloid or erythroid commitment. Serial analysis gene expression (SAGE) was employed generate four independent libraries. By analyzing differentially expressed regulated transcripts between un-stimulated stimulated cells, observed a set genes that initially up-regulated at 12h but then down-regulated 40h, exclusively after Among those found NFKB2, RELB, IL1B, LTB, LTBR, TNFRSF4, TGFB1, IKBKA. Also, inhibitor NFKBIA (IKBA) more 12h. All code signaling proteins nuclear factor kappa B pathway. NFKB2 is subunit NF-κB transcription RELB mediates non-canonical Interference RNA (RNAi) against NFKB1, control RNAi transfected into bone marrow CD34+HSPC. The percentage size colonies derived from decreased inhibition NFKB2. Altogether, our results indicate has role commitment CD34+HSPC towards lineage.
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