Distribution of Xylella fastidiosa in Citrus Rootstocks and Transmission of Citrus Variegated Chlorosis Between Sweet Orange Plants Through Natural Root Grafts

摘要: To study translocation of Xylella fastidiosa to citrus rootstocks, budsticks from variegated chlorosis (CVC)-affected cv. Pera sweet orange (Citrus sinenesis (L.) Osb.) were top grafted on 15 rootstocks. Disease symptoms conspicuous 3 months later all rootstocks tested. The presence X. was confirmed by light microscopy, double-antibody sandwich enzyme-linked immunosorbent assays, and polymerase chain reaction in rootlets main roots CVC-symptomatic 11 the These results suggest that bacterial aerial plant parts root system occurs but is not essential for induce parts. Bacterial correlated with CVC leaf-symptom severity scion. determine if disease could be transmitted natural grafts, two matched seedlings each four cultivars (Pera, Natal, Valencia, Caipira) transplanted into single pots. One seedling rootstock pair inoculated grafting a CVC-contaminated budstick while other cut graft inoculated. Transmission an noninoculated sharing same pot observed observation grafts between plants, development uninoculated plants. This first report transmission through grafts.