In vitro gene expression within membrane-free coacervate protocells

作者: T.-Y. Dora. Tang , Dirk van Swaay , Andrew deMello , J. L. Ross Anderson , Stephen Mann

DOI: 10.1039/C5CC04220H

关键词:

摘要: Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix polysaccharide/polypeptide coacervate.

参考文章(28)
Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda, Cell-free translation reconstituted with purified components Nature Biotechnology. ,vol. 19, pp. 751- 755 ,(2001) , 10.1038/90802
V. Noireaux, A. Libchaber, A vesicle bioreactor as a step toward an artificial cell assembly Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 17669- 17674 ,(2004) , 10.1073/PNAS.0408236101
David S Williams, Shogo Koga, C Rohaida C Hak, Animesh Majrekar, Avinash J Patil, Adam W Perriman, Stephen Mann, None, Polymer/nucleotide droplets as bio-inspired functional micro-compartments Soft Matter. ,vol. 8, pp. 6004- 6014 ,(2012) , 10.1039/C2SM25184A
Roberta Lentini, Michele Forlin, Laura Martini, Cristina Del Bianco, Amy C. Spencer, Domenica Torino, Sheref S. Mansy, Fluorescent proteins and in vitro genetic organization for cell-free synthetic biology. ACS Synthetic Biology. ,vol. 2, pp. 482- 489 ,(2013) , 10.1021/SB400003Y
T.-Y. Dora Tang, Massimo Antognozzi, James A. Vicary, Adam W. Perriman, Stephen Mann, Small-molecule uptake in membrane-free peptide/nucleotide protocells Soft Matter. ,vol. 9, pp. 7647- 7656 ,(2013) , 10.1039/C3SM50726B
Sandro Matosevic, Brian M. Paegel, Stepwise Synthesis of Giant Unilamellar Vesicles on a Microfluidic Assembly Line Journal of the American Chemical Society. ,vol. 133, pp. 2798- 2800 ,(2011) , 10.1021/JA109137S
John Crosby, Tom Treadwell, Michelle Hammerton, Konstantinos Vasilakis, Matthew P Crump, David S Williams, Stephen Mann, None, Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer–nucleotide coacervate droplets Chemical Communications. ,vol. 48, pp. 11832- 11834 ,(2012) , 10.1039/C2CC36533B
Mei Li, David C. Green, J. L. Ross Anderson, Bernard P. Binks, Stephen Mann, In vitro gene expression and enzyme catalysis in bio-inorganic protocells Chemical Science. ,vol. 2, pp. 1739- 1745 ,(2011) , 10.1039/C1SC00183C
Tereza Pereira de Souza, Alfred Fahr, Pier Luigi Luisi, Pasquale Stano, Spontaneous encapsulation and concentration of biological macromolecules in liposomes: an intriguing phenomenon and its relevance in origins of life. Journal of Molecular Evolution. ,vol. 79, pp. 179- 192 ,(2014) , 10.1007/S00239-014-9655-7
Mei Li, Xin Huang, T-Y Dora Tang, Stephen Mann, Synthetic cellularity based on non-lipid micro-compartments and protocell models Current Opinion in Chemical Biology. ,vol. 22, pp. 1- 11 ,(2014) , 10.1016/J.CBPA.2014.05.018