Biomaterial supports for colonic wall defect healing. An experimental study in the rat.

摘要: Abstract New artificial biomaterials were tested for support of gastro-intestinal tract wound healing in the rat. Double layered collagenic matrices prepared with purified collagens extracted from human placental tissues. Two types patches tested, first constituted a collagen type I + III layer covered by IV liquid phase (patch III/IV) and second IV/IV). The applied fibrin sealant to edges 1 cm diameter colonie wall defect Healing evolution was determined macroscopic, microscopic immunostaining studies. reconstitution three colonic layers achieved within 45 days without retraction or inflammatory reaction, while biomaterial resorbed. Human antibodies failed stain extracellular matrix. This failure may be consequence outdated more likely epitope alteration during extraction preparation collagens. A antibody staining scar zone showed basement membranes newly developed vessels 10 days, formed mucosa 20 days. reconstituted matrix able assist normal digestive defects as shown labelling new synthesized antibody. These findings use anastomosis. surgical approach allowing could reduce occurrence anastomotic leakage human.