Lab-in-a-syringe using gold nanoparticles for rapid immunosensing of protein biomarkers
作者： Gisele Elias Nunes Pauli , Alfredo de la Escosura-Muñiz , Claudio Parolo , Ivan Helmuth Bechtold , Arben Merkoçi
DOI: 10.1039/C4LC01123F
关键词:
摘要: We have developed a paper and gold nanoparticle (AuNP)-based lab-in-a-syringe (LIS) for immunosensing of biomarkers. This simple diagnostic device features simultaneous sampling vertical-flow operation, which means that unlike typical immunosensors, it does not suffer from any delay between detection. It can handle large-volume, low-concentration samples analysis in diverse applications (e.g. biomedical, environmental, food, etc.). Furthermore, its operating range sample concentration be tuned by simply changing the volume syringed sample, enables on-demand limits detection (LOD). The LIS contains two nitrocellulose pads: conjugate pad (which captures analyte) signals presence captured both embedded into reusable plastic cartridges. demonstrated efficiency detecting human IgG (HIgG) (LOD: 1.0 ng mL−1) prostate-specific antigen (PSA) (spiked urine samples; LOD: 1.9 mL−1). In field, used complete on-site or to obtain partially analyzed (AuNPs with subsequent detailed testing specialized laboratories.
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