Retracted Article: Macrophage-derived exosomes mediate osteosarcoma cell behavior by activating AKT signaling

摘要: Osteosarcoma is the most common type of bone tumor, which severely threatens health adolescents and young adults. Tumor-infiltrating macrophages have been shown to mediate cancer progression via extracellular vesicles. However, their potential mechanisms in osteosarcoma drug-resistance are still not yet known. The macrophage cell line THP1 was stimulated by phorbol myristate acetate (PMA) secrete exosomes. exosomes isolated from were characterized transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) a western blot. Cell proliferation determined using CCK-8. A transwell assay flow cytometry conducted detect migration apoptosis, respectively. expression levels AKT its phosphorylation status PMA-treated activated cells secreted an abundance with characteristics being less than 200 nm diameter, showing robust exosome markers CD63 CD81. THP1-derived promoted proliferation, chemical drug docetaxel both lines MG63 143B. inhibition generation knockdown ALIX clearly suppressed drug-resistance. Mechanistically, signaling inducing increased phosphorylated at serine 473 (p-AKT). inhibitor MK2206 significantly abolished exosome-mediated cells. In summary, our data demonstrated that macrophage-derived activating could be used as molecular target for treatment.