Transcriptional regulation of fatty acid biosynthesis in Streptococcus pneumoniae

摘要: The transcriptional regulation of membrane fatty acid composition in the human pathogen Streptococcus pneumoniae is distinct from systems utilized model organisms Escherichia coli and Bacillus subtilis. genes encoding components type II biosynthesis cluster at a single location within S. genome, second gene this (SPR0376) encodes transcription factor (FabT) that belongs to MarR superfamily. Derivatives strain D39 were constructed lacked functional FabT. This had significantly elevated levels saturated acids longer chain lengths than control strain, was unable grow pH 5.5 increased sensitivity detergent. Eliminating FabT function expression all fab with notable exception fabM. purified bound DNA palindrome located promoter regions fabT fabK cluster. analysis cells individual leads where physical properties controlled primarily by activity FabK, enoyl reductase, which diverts intermediates formation, contrast E. FabB, an elongation condensing enzyme, pulls pathway direction unsaturated synthesis.