A simplified glucagon immunoassay and its use in a study of incubated pancreatic islets.
作者： K. Nonaka , P. P. Foa
DOI: 10.3181/00379727-130-33549
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摘要: SummaryThis paper describes a rapid and sensitive radio-immunoassay for glucagon, in which powdered cellulose suspension is used the separation of free from antibody-bound glucagon. One hundred samples can be assayed conveniently 2 days. The method was applied to assay glucagon serum incubation media. Isolated rat pancreatic islets incubated Krebs—Henseleit buffer containing glucose at concentration 300 mg/100 ml secreted an average rate 42 ng/10 islets/hr. When 30 secretion 72 islets/hr (p< 0.005).
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