Proliferation of DBLOX Peroxidase-Expressing Oenocytes Maintains Innate Immune Memory in Primed Mosquitoes

摘要: Abstract Immune priming in Anopheles gambiae mosquitoes following infection with Plasmodium parasites is mediated by the systemic release of a hemocyte differentiation factor (HDF), complex lipoxin A4 bound to Evokin, lipid carrier. HDF increases proportion circulating granulocytes and enhances mosquito cellular immunity. We found that Evokin constitutively produced hemocytes fat-body cells, but expression response infection. Insects synthesize lipoxins, lack lipoxygenases. Here, we show Double Peroxidase (DBLOX) enzyme, present insects not vertebrates, essential for synthesis. DBLOX highly expressed oenocytes fat body tissue, these cells proliferate challenge. provide direct evidence modifications histone acetyltransferase AgTip60 (AGAP01539) are sustained oenocyte proliferation, synthesis immune priming. propose function as population “memory” continuously orchestrate maintain broad, long-lasting state enhanced surveillance.