Influence of age, diabetes, and cataract on calcium, lipid-calcium, and protein-calcium relationships in human lenses.

摘要: Purpose Calcium is elevated in most cataractous human lenses and may contribute to cataractogenesis. In this study, age-related changes were examined the total calcium content of clear binding lens lipids proteins. Methods Total was determined by atomic absorption spectroscopy. measured light scattering measurement spectroscopy bound unbound fractions. Results The decreased between 18 55 years age increased 75 years, as well presence cataract. levels from subjects with insulin-dependent diabetes did not differ that age-matched control subjects. vitro studies have shown can bind nearly all present lens. Age cataract diminished capacity calcium. Calcium-induced light-scattering, for proteins, correlated increasing Conclusions data support hypothesis intracellular concentrations a initiate cascade events culminates light-scattering especially lipid membranes cannot directly lenses. It has been suggested diffusible intercellular spaces outer leaflet bilayer If so, could account 150-fold difference free