Phosphorylation of the retinoblastoma-related protein p130 in growth-arrested cells.
作者： Alfredo J Canhoto , Anton Chestukhin , Larisa Litovchick , James A DeCaprio
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摘要: The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This is upon activity cyclin D/cdk4. In contrast pRB p107, phosphorylated G0 early G1 cycle. We observed that specifically on serine threonine residues in T98G cells arrested by serum deprivation or density arrest. Identification phospho-serine phospho-threonine revealed most were clustered within a short co-linear region unique p130, defined as Loop. Deletion Loop resulted change status under growth arrest conditions. Notably, deletion did not affect ability bind E2F-4 SV40 Large T antigen, induce Saos-2 cells, become hyperphosphorylated proliferative specific manner distinguishes it from p107.
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