作者: Mohar Singh , Maneesha S Saxena , Akhilesh K Tyagi , Deepak Bajaj , Kailash C Bansal
DOI: 10.1371/JOURNAL.PONE.0107484
关键词:
摘要: Characterization of natural allelic diversity and understanding the genetic structure linkage disequilibrium (LD) pattern in wild germplasm accessions by large-scale genotyping informative microsatellite single nucleotide polymorphism (SNP) markers is requisite to facilitate chickpea improvement. Large-scale validation high-throughput genome-wide physically mapped 478 genic genomic 380 transcription factor gene-derived SNP using gel-based assay, fluorescent dye-labelled automated fragment analyser matrix-assisted laser desorption ionization-time flight (MALDI-TOF) mass array have been performed. Outcome revealed their high success rate (97.5%) existence a level among 94 cultivated Cicer accessions. High intra- inter-specific polymorphic potential wider molecular (11–94%) along with broader base (13–78%) specifically functional regions was assayed markers. It suggested utility monitoring introgression transferring target trait-specific (gene) from gene pool for enhancement. Distinct species/gene pool-wise differentiation, admixed domestication pattern, differential recombination LD estimates/decay observed six structured population further signifies usefulness genetics, genomics breeding.