In TFIIH, XPD Helicase Is Exclusively Devoted to DNA Repair
作者： Jochen Kuper , Cathy Braun , Agnes Elias , Gudrun Michels , Florian Sauer
DOI: 10.1371/JOURNAL.PBIO.1001954
关键词:
摘要: The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations human are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, trichothiodystrophy. We performed a comparative analysis from Homo sapiens Chaetomium thermophilum (a closely related thermostable fungal orthologue) to decipher the different molecular prerequisites necessary for either or DNA repair. In vitro vivo assays demonstrate that mutations 4Fe4S cluster domain abrogate NER function do not affect its transcriptional activity. show p44-dependent activation promoted by stimulation ATPase Furthermore, we clearly requires binding, ATPase, activity NER. contrast, these enzymatic properties dispensable initiation. thus exclusively devoted merely acts structural scaffold maintain integrity during transcription.
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