Physiological and genetic investigations of iron deficiency in field peas (Pisum sativum L.).

摘要: Iron (Fe) deficiency chlorosis affects both yield and quality of many species, including cool-season food legumes the symptom is especially prevalent in crops grown on calcareous soils which are widely distributed southern region Australia. Although Fe fertilizers have been used to correct effective for short term control, cultivation tolerant cultivars could reduce damage long all sensitive field peas. The present study was conducted investigate various aspects genetic tolerance pea Santi Parafield, particular, with objective providing information implement an efficient breeding strategy long-term control chlorosis. Methods screen peas were developed by utilizing solution pot soil cultures. Nutrient a high concentration (10 mM) bicarbonate (HCO3⁻) either sodium (Na) or potassium (K) forms induced symptoms it possible discriminate between genotypes. Plants NaHCO₃ indicative Na toxicity therefore KHCO₃ selected culture studies. On basis this result, 37 accessions screened containing 10 mM eight that representative range response variation plant morphologies physiological These included Santi, Px-95-183-7-1, Px-89-82-1 Px-97-58-1 (tolerant genotypes) Glenroy, Px-97-9-4, Px-96-83-1-1 (moderately genotypes). Three cultivars, namely Glenroy Parafield pots identify effect three types obtained from Wangary, Millicent UC as symptoms. Severe plants Wangary most severe Parafield. Imposing moisture treatment 120% capacity more than 100% 80% capacity, treatments sensitive, intermediate remained green. chelates Fe-EDDHA Fe-EDTA applied foliar plants, soil, exhibiting All combinations fertilizer type x method application reducing shoot top leaves at time also subsequent growth, indicating leaf due deficiency. mechanism controlling chlorosis, derived backcross lines investigated. major not related acquisition Fe(III) reductase activity…