Gene alteration in zebrafish exposed to a mixture of substances of abuse

摘要: Abstract A recent surge in the use and abuse of diverse prescribed psychotic illicit drugs necessitates surveillance drug residues source water associated ecological impacts chronic exposure to aquatic organism. Thirty-six were determined discharged wastewater from two centralized municipal treatment facilities receiving creeks for seven consecutive days Kentucky. Zebrafish (Danio rerio) larvae exposed environmental relevant mixtures all residues, drugs, drugs. The extracted RNA fish homogenates was sequenced, differentially expressed sequences analyzed known or predicted nervous system expression, screened annotated protein-coding genes true cocktail mixture. Illicit stimulant (cocaine one metabolite), opioids (methadone, methadone metabolite, oxycodone), hallucinogen (MDA), benzodiazepine (oxazepam temazepam), carbamazepine, target selective serotonin reuptake inhibitors including sertraline, fluoxetine, venlafaxine, citalopram quantified 100% collected samples both creeks. high dose mixture group revealed largest genes: 100 upregulated 77 downregulated (p ≤ 0.05; q ≤ 0.05). top 20 each comprise 82 unique transcripts corresponding 74% genes, 7% non-coding sequences, 19% uncharacterized sequences. Among 61 that corresponded 23 (38%) their homologs are be other organisms. Several primarily with immune system, several major histocompatibility complex class I interferon-induced proteins. Interleukin-1 beta (downregulated this study) abnormalities considered a risk factor psychosis. This is first study assess contributions multiple classes combination developmental gene expression.