Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.

摘要: Experiments on the host cell spectrum of bovine leukaemia virus (BLV), a retrovirus closely related to human T-cell (HTLV), have yielded conflicting data. Currently, BLV is known infect B cells, whereas its ability other types, e.g. monocytes/macrophages, doubtful. As monocytes/macrophages may profound effects diversity response, we studied possibility in vitro infection, using monocytes and SV40-transformed macrophages. Proviral DNA was detected by nested polymerase chain reaction (PCR) from day 1 until end experiments at either 5 or 80, depending quantity used for infection. In addition, infection associated with morphological changes infected cells as revealed electron microscopy. The did not significantly change expression surface antigens (CD11b, CD32, major histocompatibility complex (MHC) class II) amounts cytokine transcripts (interleukin (IL)-1beta, tumour necrosis factor (TNF)-alpha, IL-6 IL-12p40) without lipopolysaccharide (LPS) stimulation. data suggest that can monocytes, but does seem influence function phenotype these cells. Infected may, however, play role viral reservoir vivo.