Submerged fermentation of wheat bran by Aspergillus flavus for production and characterization of carboxy methyl cellulase
作者： D Gomathi , C Muthulakshmi , D Guru Kumar , G Ravikumar , M Kalaiselvi
DOI: 10.1016/S2221-1691(12)60132-4
关键词:
摘要: Abstract Objective To elucidate the capabilities of carboxy methyl cellulase (CMCase) production with selective species Aspergillus flavus (A. flavus) . Methods The expression in A. was evaluated under different environmental conditions using submerged fermentation (SmF) on agricultural byproducts. Results produced high levels CMCase optimized culture 3 rd day incubation at an optimum pH 6.0, temperature 30 °C, inoculum size 4% Czapek Dox wheat bran as a substrate by SmF. Enhanced occurred addition and 1% peptone nutritional factors. enzyme hydrolyzed exhibiting V max value 40 U/mg K m 0.5 mg/mL 7. optimally activated 50 °C. Except Mg 2+ , all other metal ions such Zn Cu Na + Ca were found to be activators for activity. Conclusion kinetic parameters showed thermo stability, stability which reflects potential commercial significance enzyme.
sci-hub.se 参考文章(28)
Rajeev K Sukumaran, Reeta Rani Singhania, Ashok Pandey, Microbial cellulases ─ Production, applications and challenges Journal of Scientific & Industrial Research. ,vol. 64, pp. 832- 844 ,(2005)
S. P. Gautam, P. S. Bundela, A. K. Pandey, Jamaluddin Khan, M. K. Awasthi, S. Sarsaiya, Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnology Research International. ,vol. 2011, pp. 810425- 810425 ,(2011) , 10.4061/2011/810425
Ikram-ul- Haq, Kiran Shahzadi ., Uzma Hameed ., Muhammad Mohsin Jave ., M.A. Qadeer ., Solid-state fermentation of cellulases by locally isolated Trichoderma harzianum for the exploitation of agricultural byproducts Pakistan Journal of Biological Sciences. ,vol. 9, pp. 1779- 1782 ,(2006) , 10.3923/PJBS.2006.1779.1782
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
U. S. Puntambekar, Cellulase production by the edible mushroom Volvariella diplasia. World Journal of Microbiology & Biotechnology. ,vol. 11, pp. 695- 695 ,(1995) , 10.1007/BF00361023
Xiao-Bin Yu, Joo-Heon Nam, Hyun Shik Yun, Yoon-Mo Koo, Optimization of cellulase production in batch fermentation byTrichoderma reesei Biotechnology and Bioprocess Engineering. ,vol. 3, pp. 44- 47 ,(1998) , 10.1007/BF02932483
D. Swaroopa Rani, Sharmila Thirumale, Krishna Nand, Production of Cellulase by Clostridium Papyrosolvens CFR-703 World Journal of Microbiology & Biotechnology. ,vol. 20, pp. 629- 632 ,(2004) , 10.1023/B:WIBI.0000043193.39486.32
Hans Lineweaver, Dean Burk, The Determination of Enzyme Dissociation Constants Journal of the American Chemical Society. ,vol. 56, pp. 658- 666 ,(1934) , 10.1021/JA01318A036
Shunichi Akiba, Yoshiharu Kimura, Kenji Yamamoto, Hidehiko Kumagai, Purification and characterization of a protease-resistant cellulase from Aspergillus niger Journal of Fermentation and Bioengineering. ,vol. 79, pp. 125- 130 ,(1995) , 10.1016/0922-338X(95)94078-6
A.A. Sherief, A.B. El-Tanash, N. Atia, Cellulase Production by Aspergillus fumigatus Grown on Mixed Substrate of Rice Straw and Wheat Bran Research Journal of Microbiology. ,vol. 5, pp. 199- 211 ,(2010) , 10.3923/JM.2010.199.211