Thermally induced osteocyte damage initiates a remodelling signaling cascade.

摘要: Thermal elevations experienced by bone during orthopaedic procedures, such as cutting and drilling, exothermal reactions from cement, thermal therapies tumor ablation, can result in damage leading to death of native cells (osteocytes, osteoblasts, osteoclasts mesenchymal stem cells). Osteocytes are believed be the orchestrators remodeling, which recruit nearby osteoclast osteoblasts control resorption growth response mechanical stimuli physical damage. However, whether heat-induced osteocyte directly elicit remodelling has yet determined. This study establishes link between remodeling cascade. We show that osteocytes exposed (47°C for 1 minute) become significantly apoptotic alter expression osteogenic genes (Opg Cox2). The Rankl/Opg ratio is consistently down-regulated, at days 1, 3 7 MLO-Y4s heat-treated 47°C minute. Additionally, pro-osteoblastogenic signaling marker Cox2 up-regulated day 7. Furthermore, secreted factors administered MSCs using a novel co-culture system shown activate pre-osteoblastic increase production pro-osteoblastic differentiation marker, alkaline phosphatase (day 7, 14), calcium deposition 21). Most interestingly, an initial pro-osteoclastogenic (increase Rankl 1) followed later stage (down-regulation up-regulation Opg 7) was observed non-heat-treated when these were biochemicals produced MLO-Y4s. Taken together, results elucidate vital role detecting responding means thermally induced apoptosis cascade responses.