FACTORS AFFECTING THE HEMOLYTIC ACTION OF "LYSOLECITHIN" UPON RABBIT ERYTHROCYTES

摘要: 1. Lysolipid was prepared by the action of snake venom on egg yolk, and a study made factors affecting its hemolytic upon rabbit erythrocytes. 2. Lysis proceeded very rapidly at first, then ceased within few minutes room temperature. A given amount lysin appeared to hemolyze fixed number cells, under specified conditions. 3. The more dilute erythrocyte suspensions required relatively per cell, for 50 cent hemolysis suspension. There may be an equilibrium between dissolved in medium that adsorbed cells. 4. degree varying concentrations measured, cells showed typical distribution resistance hemolysis. 5. As temperature lowered lysis extensive. Adsorption cell surface apparently increased. 6. erythrocytes increased slightly as pH raised from 5.5 7.8. 7. Resistance independent tonicity initial volume. magnitude probably determining factor. 8. marked shrinkage observed presence calcium ions lysin, but not absence lysin. 9. Hemolytic curves obtained Wilbrandt technique were "colloid-osmotic" type. However, there no evidence prolytic loss potassium ions. 10. Hypotonic fragility rate penetration thiourea greatly