Retinal adhesiveness is weakened by enzymatic modification of the interphotoreceptor matrix in vivo.

摘要: The role of interphotoreceptor matrix (IPM) constituents in mediating adhesion between the retina and retinal pigment epithelium (RPE) was investigated by injecting specific enzymes into rabbit eyes either intravitreally or subretinally. Retinal adhesiveness measured peeling from 1-3 days later observing amount adherent pigment. Effects on IPM were monitored observation peanut agglutinin (PNA) binding to cone sheaths; RPE toxicity excluded electroretinography histology. Three that degrade glycosaminoglycans saccharides known be (chondroitinase ABC, neuraminidase, testicular hyaluronidase) both weakened altered PNA binding, although effects sheaths different for each enzyme. An enzyme hyaluronic acid (Streptomyces-derived hyaluronidase), which has not been identified as a major constituent, had no effect binding. authors conclude IPM-associated glycoconjugates participate adhesion, their precise composition, interaction with components, relationship other mechanisms remain determined.