Acid and Alkaline Phosphatase in Serum: Two-point method

摘要: Publisher Summary This chapter elaborates on phosphatases that catalyze the hydrolytic cleavage of phosphoric acid esters. Alkaline occur in practically all animal and human tissues. Acid are also found nearly cells. The older methods assay use amount phosphate liberated from a substrate as an index phosphatase activity. 4-nitrophenol per unit time, determined alkaline solution at 400–420 nm, is measure reaction stopped by addition NaOH. normal men women have highest activity with broad optimum between pH 5.5 6.1. On other hand prostatic has relatively high 4.8. water content nitrophenylphosphate not constant commercial preparations therefore should be checked. buffer/substrate solutions stored 0–4°C; they stable for 1 week. continuous measurements advantage only one pipetting necessary can carried out rapidly.