Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-Gi
作者： F.F. Chu , J.H. Doroshow , R.S. Esworthy
DOI: 10.1016/S0021-9258(18)53812-6
关键词:
摘要: We have characterized a new selenium-dependent glutathione peroxidase, GSHPx-GI, by expressing a GSHPx-GI cDNA isolated from human hepatoma HepG2 cells in human mammary carcinoma MCF-7 cells, which have virtually undetectable expression of either the classical cellular enzyme, GSHPx-1, or GSHPx-GI at the protein level. One of the G418-resistant clones, neo-D1, expresses the transfected GSHPx-GI cDNA. This is based on 1) the presence of an additional GSHPx-GI DNA restriction fragment detected by Southern …
sci-hub.st 参考文章(33)
M Maiorino, F F Chu, F Ursini, K J Davies, J H Doroshow, R S Esworthy, Phospholipid hydroperoxide glutathione peroxidase is the 18-kDa selenoprotein expressed in human tumor cell lines. Journal of Biological Chemistry. ,vol. 266, pp. 7728- 7732 ,(1991) , 10.1016/S0021-9258(20)89509-X
J P Thomas, M Maiorino, F Ursini, A W Girotti, Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation. In situ reduction of phospholipid and cholesterol hydroperoxides. Journal of Biological Chemistry. ,vol. 265, pp. 454- 461 ,(1990) , 10.1016/S0021-9258(19)40252-4
Nalini N. Vilas, R. Raines Bell, H. H. Draper, Influence of Dietary Peroxides, Selenium and Vitamin E on Glutathione Peroxidase of the Gastrointestinal Tract Journal of Nutrition. ,vol. 106, pp. 589- 596 ,(1976) , 10.1093/JN/106.5.589
Nelly Avissar, J. Randall Slemmon, Ivan S. Palmer, Harvey J. Cohen, Partial sequence of human plasma glutathione peroxidase and immunologic identification of milk glutathione peroxidase as the plasma enzyme. Journal of Nutrition. ,vol. 121, pp. 1243- 1249 ,(1991) , 10.1093/JN/121.8.1243
Shinichi Yoshimura, Keiichi Watanabe, Hiroshi Suemizu, Takashi Onozawa, Junzou Mizoguchi, Ken Tsuda, Hajime Hatta, Tetsuya Moriuchi, Tissue Specific Expression of the Plasma Glutathione Peroxidase Gene in Rat Kidney1 The Journal of Biochemistry. ,vol. 109, pp. 918- 923 ,(1991) , 10.1093/OXFORDJOURNALS.JBCHEM.A123480
Kazuhiko Takahashi, Masami Akasaka, Yutaka Yamamoto, Chigako Kobayashi, Junzo Mizoguchi, Jiro Koyaxna, Primary structure of human plasma glutathione peroxidase deduced from cDNA sequences Journal of Biochemistry. ,vol. 108, pp. 145- 148 ,(1990) , 10.1093/OXFORDJOURNALS.JBCHEM.A123172
Wolfgang A. GÜNZLER, Gerd J. STEFFENS, Adelbert GROSSMANN, Sung-Man A. KIM, Fritz ÖTTING, Albrecht WENDEL, Leopold FLOHÉ, The amino-acid sequence of bovine glutathione peroxidase. Biological Chemistry. ,vol. 365, pp. 195- 212 ,(1984) , 10.1515/BCHM2.1984.365.1.195
Sunil Chada, Michelle M. Le Beau, Linda Casey, Peter E. Newburger, Isolation and chromosomal localization of the human glutathione peroxidase gene. Genomics. ,vol. 6, pp. 268- 271 ,(1990) , 10.1016/0888-7543(90)90566-D
Richard C. Mulligan, Bruce H. Howard, Paul Berg, Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome Nature. ,vol. 277, pp. 108- 114 ,(1979) , 10.1038/277108A0
Guy T. Mullenbach, Azita Tabrizi, Bruce D. Irvine, Graeme I. Bell, Robert A. Hallewell, Sequence of a cDNA coding for human glutathione peroxidase confirms TGA encodes active site selenocysteine Nucleic Acids Research. ,vol. 15, pp. 5484- 5484 ,(1987) , 10.1093/NAR/15.13.5484