Tetrandrine inhibits proliferation of colon cancer cells by BMP9/ PTEN/ PI3K/AKT signaling

摘要: Abstract Despite advances in screening and treatment, colon cancer remains one of the leading causes cancer-related death. Finding novel useful drug treatment targets is also an urgent need for clinical applications. Tetrandrine (Tet) extracted from Chinese medicinal herbal medicine, which a well-known calcium blocker with variety pharmacological activities, including anti-cancer. In this study, we recruited cell viability assay, flow cytometry analysis, cloning formation to confirm that Tet can inhibit proliferation SW620 cells, induce apoptosis. Mechanically, confirmed up-regulates mRNA protein level BMP9 SW620 cells. Over-expression enhances anti-cancer effects but these be partly reversed by silencing BMP9. Also, reduces phosphorylation Aktl/2/3 could elevated overexpressed impaired Furthermore, demonstrated phosphorylated PTEN, promoted BMP9, analogously attenuated through Finally, introduced xenograft tumor model investigate anti-proliferative effect Tet, further explore PTEN Our findings suggested activity SW620 cells may mediated up-regulating followed inactivation PI3K/Akt at least.