Degradation of substituted indoles by an indole-degrading methanogenic consortium.

摘要: Degradation of indole by an indole-degrading methanogenic consortium enriched from sewage sludge proceeded through a two-step hydroxylation pathway yielding oxindole and isatin. The ability this to hydroxylate subsequently degrade substituted indoles was investigated. Of the tested, able transform or 3-methylindole 3-indolyl acetate. Oxindole, 3-methyloxindole, indoxyl were identified as metabolites indole, 3-methylindole, acetate degradation, respectively. Isatin (indole-2,3-dione) produced intermediate when amended with oxindole, providing evidence that degradation successive 2- 3-positions prior ring cleavage between C-2 C-3 atoms on pyrrole indole. presence methyl group (-CH3) at either 1- 2-position inhibited initial reaction. hydroxylated in but not 3-position could be further metabolized oxindole-isatin pathway. Indoxyl (indole-3-one), deacetylated product acetate, thus consortium. When H atom electron-donating (i.e., -CH3) present 3-position, 2-position, electron-withdrawing substituent groups -OH -COOH) hydroxylation.